Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification
نویسندگان
چکیده
منابع مشابه
Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification.
PCR analysis of 16S-23S internal transcribed spacer (PCR ribotyping) and tRNA intergenic spacer (tDNA-PCR) were evaluated for their effectiveness in identification of clinical strains of Klebsiella pneumoniae and differentiation with related species. For this purpose both methods were applied to forty-three clinical isolates biochemically identified as K. pneumoniae subsp. pneumoniae isolated f...
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The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different species. It was concluded that interl...
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Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for ...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2007
ISSN: 1678-8060,0074-0276
DOI: 10.1590/s0074-02762007005000113